Journal: Nutrition & Diabetes

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice

doi: 10.1038/nutd.2014.4

Figure Lengend Snippet: ApoA-I −/− mice have lower expression of adipose tissue lipase (ATGL), decreased p-HSL and differentially expressed lipid metabolism genes in white adipose tissue (WAT) compared with WT mice during caloric restriction. Animals were fed the OD and subjected to caloric restriction as described in the legend of . WAT mRNA levels of lipase and lipid metabolism genes were quantified by reverse transcriptase-PCR, and cell lysates were immunoblotted for p-HSL, HSL and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as described in Materials and Methods section. ( a ) Immunoblots for p-HSL, HSL and GAPDH in WAT of WT and apoA-I −/− mice. Data presented are from three representative animals in each group. ( b ) Densitometric data for p-HSL (calculated by pHSL/HSL) and HSL (calculated by HSL/GAPDH). Data are presented as mean±s.e.m. ( n =5). ( c ) mRNA levels of lipase and lipid metabolism genes in WAT of WT (white bar) and apoA-I −/− (gray bar) mice. Data are presented as fold change from WT mice as mean±s.e.m. ( n =17). The asterisk denotes statistically significant differences from WT mice ( P <0.05, t -test).

Article Snippet: First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers.

Techniques: Expressing, Reverse Transcription, Western Blot

Journal: Nutrition & Diabetes

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice

doi: 10.1038/nutd.2014.4

Figure Lengend Snippet: ApoA-I tg/tg mice possess higher epididymal white adipose tissue (WAT) expressions of adipose tissue lipase (ATGL) and HSL as well as greater p-HSL compared with WT mice. Animals were fed the OD as described in the legend of . Total RNA and cell lysates were prepared from epididymal WAT. mRNA levels of lipase and lipid metabolism genes were quantified by reverse transcriptase-PCR and normalized to L32 levels; cell lysate were immunoblotted for p-HSL, HSL and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as described in Materials and Methods section. ( a ) Immunoblots of p-HSL, HSL and GAPDH in WAT of WT and apoA-I tg/tg mice. Data presented are from three representative animals in each group. ( b ) Densitometric data of p-HSL (calculated by pHSL/HSL) and HSL (calculated by HSL/GAPDH). Data are presented as mean±s.e.m. ( n =5). ( c ) mRNA levels of lipase, lipid metabolism and fatty acid oxidation genes in WAT of WT (white bar) and apoA-I tg/tg (gray bar) mice. Data are presented as fold change from values of WT mice as mean±s.e.m. ( n =11). The asterisk denotes statistically significant differences from WT mice ( P <0.05, t -test).

Article Snippet: First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers.

Techniques: Reverse Transcription, Western Blot